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Methyl red – A classic pH indicator with extremely precise color change

by Biolaboratorium 27 Nov 2025

In the world of analytical chemistry, there are numerous tools and aids that help researchers and laboratory technicians perform precise measurements and gain insights. One such valuable assistant is the pH indicator methyl red, which has been used in laboratories around the world for decades.

The Importance of pH Indicators in Chemistry

pH indicators play a crucial role in chemical analysis and research. They enable the pH value of a solution to be determined quickly and easily without the need for complex measuring equipment. This value reveals whether a solution is acidic, neutral, or basic - information that is of great importance for many chemical processes and reactions.

Classic pH indicators like methyl red are colored organic compounds that take on different colors depending on the pH of the solution. The color change allows the pH value to be read quickly and precisely. For example, methyl red appears red in acidic solutions, yellow in neutral solutions, and orange to violet in basic solutions.

The Discovery and Development of Methyl Red

Methyl red belongs to the azo dyes, a class of synthetic dyes first produced in the late 1800s. The chemist Otto Nikolaus Witt discovered these dyes in 1876 and recognized their great potential for various applications.

Methyl red itself was developed in 1893 by the German chemist Rudolf Nietzki. He synthesized the dye from dimethylaniline and 2-nitrobenzoic acid and quickly recognized methyl red's suitability as a pH indicator. Thanks to its precise color changes in the pH range of 4.4 to 6.2, methyl red became one of the most commonly used laboratory indicators in the following decades.

Chemical Structure and Function of Methyl Red

The chemical structure of methyl red is relatively complex. It is an azo dye consisting of two aromatic rings and an azo group (-N=N-). Depending on the pH of the solution, the protonation of the carboxyl group changes, which in turn affects the conjugation of the dye system.

In acidic solutions (pH < 4.4), methyl red is protonated and therefore appears red. In neutral solutions (pH 4.4 - 6.2), the dye is unprotonated and shows a yellow color. In basic solutions (pH > 6.2), the carboxyl group becomes deprotonated, resulting in an orange to violet hue.

This precise color change over a relatively narrow pH range makes methyl red a very valuable tool in chemical analysis. Researchers can use methyl red to quickly and easily determine the pH of a solution without needing to use expensive measuring equipment.

Practical applications of methyl red

Methyl red is used in numerous areas of chemistry. The color indicator is particularly widespread in research laboratories and educational institutions. But it is also used in industry, such as in food production or water treatment.

Use in Analytical Chemistry

In analytical chemistry, methyl red is an indispensable tool. Researchers use the color indicator to quickly and precisely determine the pH of solutions. For example, acid-base titrations can be performed or the course of chemical reactions can be tracked.

Furthermore, methyl red can also be used as a complexing agent. In combination with heavy metal ions such as iron(III) or copper(II), the dye forms colored complexes that can in turn be used for the detection and quantification of these ions.

Use in education

Methyl red is also a widely used laboratory indicator in schools and universities. Chemistry students and those in related fields often learn how to handle pH indicators through experiments with methyl red. For example, they can observe the color change during acid-base titrations or determine the pH of unknown solutions.

The use of methyl red in education has the advantage that the color indicator works very precisely and reliably. This allows pupils and students to investigate and understand acid-base reactions and pH values very accurately.

Further applications

In addition to its use in research and education, methyl red is also used in industrial processes. For example, the dye is used in the food industry to control the pH of products such as juices or milk.

Methyl red also plays an important role in water treatment. The color indicator can be used to monitor the pH of drinking water or wastewater, thus ensuring water quality.

Conclusion: Methyl red – an indispensable helper in chemistry

Methyl red is a classic pH indicator that has been used in laboratories worldwide for over 100 years. Thanks to its precise color transition in the pH range of 4.4 to 6.2, this azo dye enables quick and reliable measurements of the acid-base behavior of solutions.

Whether in analytical research, educational institutions, or industrial processes – methyl red has established itself as an indispensable tool in chemistry. The color indicator helps scientists, students, and technicians better understand and control chemical processes.

In the future, methyl red will certainly continue to play an important role in laboratories and research institutions. This classic pH indicator offers a simple, cost-effective, and precise way to determine the pH value of solutions – an ability that remains indispensable in chemistry.

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